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MFF Monoclonal antibody

MFF Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66527-1-Ig
Clone No.1G4B12

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

AD033, C2orf33, MFF, mitochondrial fission factor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inpig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66527-1-Ig targets MFF in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MFF fusion protein Ag9990 相同性解析による交差性が予測される生物種
Full Name mitochondrial fission factor
Calculated molecular weight 38 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
RRIDAB_2881890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Protocols

Product Specific Protocols
WB protocol for MFF antibody 66527-1-IgDownload protocol
IHC protocol for MFF antibody 66527-1-IgDownload protocol
IF protocol for MFF antibody 66527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Phytother Res

Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow

Authors - Jing-Jing Li
mouseWB

J Adv Res

Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics

Authors - Liuyang Tian
mouse,humanWB

iScience

Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis

Authors - Yunfan He
mouseWB

J Alzheimers Dis

Sex-Specific Early Retinal Dysfunction in Mutant TDP-43 Transgenic Mice

Authors - Ju Gao
IF

Theriogenology

OPA1 deficiency induces mitophagy through PINK1/Parkin pathway during bovine oocytes maturation

Authors - Tiancang Han
ratIF

Respir Res

Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia

Authors - Tong Sun
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