Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
66527-1-Ig targets MFF in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
| Tested Reactivity | Human, pig, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MFF fusion protein Ag9990 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial fission factor |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 35-38 kDa |
| GenBank accession number | BC000797 |
| Gene Symbol | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_2881890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9GZY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MFF antibody 66527-1-Ig | Download protocol |
| IHC protocol for MFF antibody 66527-1-Ig | Download protocol |
| IF protocol for MFF antibody 66527-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytother Res Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow | ||
J Adv Res Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics | ||
iScience Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis | ||
Theriogenology OPA1 deficiency induces mitophagy through PINK1/Parkin pathway during bovine oocytes maturation | ||
Respir Res Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia |