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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MFF Monoclonal antibody

MFF Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4B12

Cat no : CL488-66527

Synonyms

AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated MFF Monoclonal antib (CL488-66527)

    IF Staining of mouse brain using CL488-66527

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 MFF antibody (CL488-66527, Clone: 1G4B12 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66527 targets MFF in IF-P applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MFF fusion protein Ag9990 相同性解析による交差性が予測される生物種
Full Name mitochondrial fission factor
Calculated molecular weight 38 kDa
GenBank accession numberBC000797
Gene symbol MFF
Gene ID (NCBI) 56947
RRIDAB_2919338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MFF antibody CL488-66527Download protocol
Standard Protocols
Click here to view our Standard Protocols