Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-17090 targets MFF in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MFF fusion protein Ag9990 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial fission factor |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 26-29 kDa, 35-38 kDa |
| GenBank accession number | BC000797 |
| Gene Symbol | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_2919839 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZY8 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MFF antibody CL594-17090 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |