CoraLite® Plus 488-conjugated MGMT Monoclonal antibody
MGMT Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H2C9
Cat no : CL488-67476
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67476 targets MGMT in IF applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MGMT fusion protein Ag29936 相同性解析による交差性が予測される生物種 |
Full Name | O-6-methylguanine-DNA methyltransferase |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC000824 |
Gene symbol | MGMT |
Gene ID (NCBI) | 4255 |
RRID | AB_2919482 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 MGMT antibody CL488-67476 | Download protocol |
FC protocol for CL Plus 488 MGMT antibody CL488-67476 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |