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  • Featured Product
  • KD/KO Validated

MIA3 Polyclonal antibody, PBS Only

MIA3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17481-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using MIA3 Polyclonal antibody (17481-1-AP)

    WB analysis of HEK-293T using 17481-1-AP (same clone as 17481-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MIA3 Polyclonal antibody (17481-1-AP)

    IP experiment of HeLa using 17481-1-AP (same clone as 17481-1-PBS)

    IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17481-1-PBS targets MIA3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIA3 fusion protein Ag11514 相同性解析による交差性が予測される生物種
Full Name melanoma inhibitory activity family, member 3
Calculated molecular weight214 kDa
Observed molecular weight 300 kDa
GenBank accession numberBC047116
Gene symbol MIA3
Gene ID (NCBI) 375056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.