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  • Featured Product
  • KD/KO Validated

MIA3 Polyclonal antibody, PBS Only

MIA3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17481-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using MIA3 Polyclonal antibody (17481-1-AP)

    WB analysis of HEK-293T using 17481-1-AP (same clone as 17481-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MIA3 Polyclonal antibody (17481-1-AP)

    IP experiment of HeLa using 17481-1-AP (same clone as 17481-1-PBS)

    IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MIA3 Polyclonal antibody (17481-1-AP)

    IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17481-1-PBS targets MIA3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIA3 fusion protein Ag11514 相同性解析による交差性が予測される生物種
Full Name melanoma inhibitory activity family, member 3
Calculated molecular weight214 kDa
Observed molecular weight 300 kDa
GenBank accession numberBC047116
Gene symbol MIA3
Gene ID (NCBI) 375056
RRIDAB_2878411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.