Validation Data Gallery

Filter: AllWBIHC

WB analysis using 66384-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 66384-1-Ig
Raji cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 66384-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66384-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Daudi using 66384-1-Ig
Daudi cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 66384-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66384-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 66384-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 66384-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens).

MICA Monoclonal antibody

MICA Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66384-1-Ig

Clone No.1E2C8

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

MIC A, MICA, PERB11.1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HaCaT cells, Daudi cells, Raji cells, Jurkat cells, HeLa cells, A431 cells
Positive IHC detected in	human tonsillitis tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

66384-1-Ig targets MICA in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	MICA fusion protein Ag3298 相同性解析による交差性が予測される生物種
Full Name	MHC class I polypeptide-related sequence A
Calculated molecular weight	383 aa, 43 kDa
Observed molecular weight	43-50 kDa
GenBank accession number	BC016929
Gene Symbol	MICA
Gene ID (NCBI)	100507436
RRID	AB_2881760
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q29983
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).

Protocols

Product Specific Protocols
WB protocol for MICA antibody 66384-1-Ig	Download protocol
IHC protocol for MICA antibody 66384-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Lett Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer Authors - Deli Mao View Article
human	IF	Pharmacol Res IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10. Authors - Xin Fang View Article
human,mouse	WB,IF	Phytomedicine Ginsenoside Rh2 enhances immune surveillance of natural killer (NK) cells via inhibition of ERp5 in breast cancer Authors - Chunmei Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66384-1-Ig

WB analysis of Raji using 66384-1-Ig

WB analysis of Jurkat using 66384-1-Ig

WB analysis of HeLa using 66384-1-Ig

WB analysis of Daudi using 66384-1-Ig

IHC staining of human tonsillitis using 66384-1-Ig