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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MICA Monoclonal antibody, PBS Only

MICA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2C8

Cat no : 66384-1-PBS

Synonyms

MIC A, MICA, PERB11.1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MICA Monoclonal antibody (66384-1-Ig)

    WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MICA Monoclonal antibody (66384-1-Ig)

    WB analysis of Raji using 66384-1-Ig (same clone as 66384-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MICA Monoclonal antibody (66384-1-Ig)

    WB analysis of Jurkat using 66384-1-Ig (same clone as 66384-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using MICA Monoclonal antibody (66384-1-Ig)

    WB analysis of Daudi using 66384-1-Ig (same clone as 66384-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MICA Monoclonal antibody (66384-1-Ig)

    WB analysis of HeLa using 66384-1-Ig (same clone as 66384-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MICA Monoclonal antibody (66384-1-Ig)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MICA Monoclonal antibody (66384-1-Ig)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MICA Monoclonal antibody (66384-1-Ig)

    IHC staining of human colon using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MICA Monoclonal antibody (66384-1-Ig)

    IHC staining of human colon using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66384-1-PBS targets MICA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MICA fusion protein Ag3298 相同性解析による交差性が予測される生物種
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight 43-50 kDa
GenBank accession numberBC016929
Gene symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_2881760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).