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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

6D4

Cat no : APC-65161

Synonyms

MICA, MICB

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using APC Anti-Human MICA/MICB (6D4) (APC-65161)

    FC experiment of HeLa using APC-65161

    1X10^6 HeLa cells were surface stained with 5 ul APC Anti-Human MICA/MICB (APC-65161, Clone:6D4) or APC Mouse IgG2a Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of HeLa cells using APC Anti-Human MICA/MICB (6D4) (APC-65161)

    FC experiment of HeLa using APC-65161

    1X10^6 HeLa cells were surface stained with 5 ul APC Anti-Human MICA/MICB (APC-65161, Clone:6D4) or APC Mouse IgG2a Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65161 targets MICA/MICB in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Transfected C1R cells expressing MICA 相同性解析による交差性が予測される生物種
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight 383 aa, 43 kDa
GenBank accession numberBC016929
Gene symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_2883034
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Protocols

Product Specific Protocols
FC protocol for APC MICA/MICB antibody APC-65161Download protocol
Standard Protocols
Click here to view our Standard Protocols