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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MICAL1 Monoclonal antibody

MICAL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3F5D4

Cat no : 68297-1-Ig

Synonyms

NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, 3F5D4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MICAL1 Monoclonal antibody (68297-1-Ig)

    WB analysis using 68297-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using MICAL1 Monoclonal antibody (68297-1-Ig)

    WB analysis of HSC-T6 using 68297-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using MICAL1 Monoclonal antibody (68297-1-Ig)

    WB analysis of RAW 264.7 using 68297-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MICAL1 Monoclonal antibody (68297-1-Ig)

    IP experiment of HeLa using 68297-1-Ig

    IP result of anti-MICAL1 (IP:68297-1-Ig, 4ug; Detection:68297-1-Ig 1:2000) with HeLa cells lysate 1720 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MICAL1 Monoclonal antibody (68297-1-Ig)

    IF Staining of HeLa using 68297-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MICAL1 antibody (68297-1-Ig, Clone: 3F5D4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

  • Flow cytometry (FC) experiment of HeLa cells using MICAL1 Monoclonal antibody (68297-1-Ig)

    FC experiment of HeLa using 68297-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MICAL1 (68297-1-Ig, Clone:3F5D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, RAW 264.7 cells, HSC-T6 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68297-1-Ig targets MICAL1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MICAL1 fusion protein Ag6578 相同性解析による交差性が予測される生物種
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 1
Calculated molecular weight 118 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC052983
Gene symbol MICAL1
Gene ID (NCBI) 64780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

Protocols

Product Specific Protocols
WB protocol for MICAL1 antibody 68297-1-IgDownload protocol
IF protocol for MICAL1 antibody 68297-1-IgDownload protocol
IP protocol for MICAL1 antibody 68297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols