CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody
MIS12 Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G5F6
Cat no : CL488-67475
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells, HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67475 targets MIS12 in WB, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MIS12 fusion protein Ag16826 相同性解析による交差性が予測される生物種 |
Full Name | MIS12, MIND kinetochore complex component, homolog (yeast) |
Calculated molecular weight | 205 aa, 24 kDa |
Observed molecular weight | 25 kDa |
GenBank accession number | BC000229 |
Gene symbol | MIS12 |
Gene ID (NCBI) | 79003 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CL Plus 488 MIS12 antibody CL488-67475 | Download protocol |
FC protocol for CL Plus 488 MIS12 antibody CL488-67475 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |