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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated MIS12 Monoclonal ant (CL488-67475)

    WB analysis using CL488-67475

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67475 (MIS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MIS12 Monoclonal ant (CL488-67475)

    FC experiment of HeLa using CL488-67475

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MIS12 (CL488-67475, Clone:2G5F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody

MIS12 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67475
Clone No.2G5F6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67475 targets MIS12 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MIS12 fusion protein Ag16826 相同性解析による交差性が予測される生物種
Full Name MIS12, MIND kinetochore complex component, homolog (yeast)
Calculated molecular weight 205 aa, 24 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC000229
Gene Symbol MIS12
Gene ID (NCBI) 79003
RRIDAB_2934544
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H081
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
FC protocol for CL Plus 488 MIS12 antibody CL488-67475Download protocol
Standard Protocols
Click here to view our Standard Protocols