Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, 4T1 cells, PC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, mMSCs cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 86 publications below |
| IHC | See 11 publications below |
| IF | See 12 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
66675-1-Ig targets MLKL in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MLKL fusion protein Ag15188 相同性解析による交差性が予測される生物種 |
| Full Name | mixed lineage kinase domain-like |
| Calculated molecular weight | 471 aa, 54 kDa |
| Observed molecular weight | 35-40 kDa, 50-55 kDa |
| GenBank accession number | BC028141 |
| Gene Symbol | MLKL |
| Gene ID (NCBI) | 197259 |
| RRID | AB_2882029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NB16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MLKL antibody 66675-1-Ig | Download protocol |
| IHC protocol for MLKL antibody 66675-1-Ig | Download protocol |
| IF protocol for MLKL antibody 66675-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Pressure Drives Rapid Burst-Like Coordinated Cellular Motion from 3D Cancer Aggregates. | ||
Acta Pharm Sin B Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy | ||
Acta Pharm Sin B A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein. | ||
Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis. | ||
Cell Death Dis S100A8/A9hi neutrophils induce mitochondrial dysfunction and PANoptosis in endothelial cells via mitochondrial complex I deficiency during sepsis | ||
Free Radic Biol Med Short-term exposure to low doses of aflatoxin B1 aggravates nonalcoholic steatohepatitis by TLR4-mediated necroptosis |