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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLKL Monoclonal antibody (66675-1-Ig)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MLKL Monoclonal antibody (66675-1-Ig)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using MLKL Monoclonal antibody (66675-1-Ig)

    WB analysis of 4T1 using 66675-1-Ig (same clone as 66675-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using MLKL Monoclonal antibody (66675-1-Ig)

    IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLKL Monoclonal antibody (66675-1-Ig)

    IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (66675-1-Ig, Clone: 3D4C6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MLKL Monoclonal antibody, PBS Only

MLKL Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66675-1-PBS
Clone No.3D4C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Mixed lineage kinase domain-like protein, hMLKL, 3D4C6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66675-1-PBS targets MLKL in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188 相同性解析による交差性が予測される生物種
Full Name mixed lineage kinase domain-like
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight35-40 kDa, 50-55 kDa
GenBank accession numberBC028141
Gene Symbol MLKL
Gene ID (NCBI) 197259
RRIDAB_2882029
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NB16
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).