Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, pig brain tissue, PC-3 cells, Jurkat cells, human brain tissue |
| Positive IHC detected in | human kidney tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
66092-1-Ig targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MLPH fusion protein Ag19286 相同性解析による交差性が予測される生物種 |
| Full Name | melanophilin |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC001653 |
| Gene Symbol | MLPH |
| Gene ID (NCBI) | 79083 |
| RRID | AB_11232039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BV36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MLPH antibody 66092-1-Ig | Download protocol |
| IHC protocol for MLPH antibody 66092-1-Ig | Download protocol |
| IF protocol for MLPH antibody 66092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects | ||
Mol Med Rep Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes. |