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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLPH Monoclonal antibody, PBS Only

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A8D12

Cat no : 66092-1-PBS

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of pig brain using 66092-1-Ig (same clone as 66092-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of HEK-293 using 66092-1-Ig (same clone as 66092-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of PC-3 using 66092-1-Ig (same clone as 66092-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLPH Monoclonal antibody (66092-1-Ig)

    IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66092-1-PBS targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLPH fusion protein Ag19286 相同性解析による交差性が予測される生物種
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_11232039
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.