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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLPH Recombinant antibody (84282-1-RR)

    WB analysis using 84282-1-RR (same clone as 84282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01196-3

    Sandwich ELISA standard curve of MP01196-3

    Sandwich ELISA standard curve of MP01196-3, Human MLPH Recombinant Matched Antibody Pair - PBS only. 84282-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32931. 84282-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01196-3

    Sample test of MP01196-3

    The mean MLPH concentration was determined to be 5.9 ng/mL in A549 cell extract based on a 1.5 mg/mL extract load and 1.7 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84282-1-RR

    Affinity and Kinetic Characterization of 84282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

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MLPH Recombinant antibody, PBS Only (Capture)

MLPH Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84282-1-PBS
Clone No.241621G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241621G4, Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84282-1-PBS targets MLPH as part of a matched antibody pair:

MP01196-3: 84282-1-PBS capture and 84282-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MLPH fusion protein Ag32931 相同性解析による交差性が予測される生物種
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight66 kDa, 80 kDa
GenBank accession numberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV36
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.