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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLPH Recombinant antibody

MLPH Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241621G4

Cat no : 84282-1-RR

Synonyms

SLAC2A, SLAC2 A, Melanophilin, Exophilin-3, Exophilin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLPH Recombinant antibody (84282-1-RR)

    WB analysis using 84282-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Recombinant antibody (84282-1-RR)

    IHC staining of human skin cancer using 84282-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84282-1-RR

    Affinity and Kinetic Characterization of 84282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, PC-3 cells, HT-29 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84282-1-RR is MLPH Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MLPH fusion protein Ag32931 相同性解析による交差性が予測される生物種
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight66 kDa, 80 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Protocols

Product Specific Protocols
WB protocol for MLPH antibody 84282-1-RRDownload protocol
IHC protocol for MLPH antibody 84282-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols