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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MMAB Polyclonal antibody

MMAB Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11137

Synonyms

EC:2.5.1.-, Corrinoid adenosyltransferase MMAB, cblB, ATR, ATP:co(I)rrinoid adenosyltransferase MMAB

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated MMAB Polyclonal anti (CL488-11137)

    IF Staining of HeLa using CL488-11137

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MMAB antibody (CL488-11137) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MMAB Polyclonal anti (CL488-11137)

    FC experiment of HeLa using CL488-11137

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated MMAB Polyclonal antibody (CL488-11137)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11137 targets MMAB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMAB fusion protein Ag1602 相同性解析による交差性が予測される生物種
Full Name methylmalonic aciduria (cobalamin deficiency) cblB type
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC011831
Gene symbol MMAB
Gene ID (NCBI) 326625
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MMAB antibody CL488-11137Download protocol
FC protocol for CL Plus 488 MMAB antibody CL488-11137Download protocol
Standard Protocols
Click here to view our Standard Protocols