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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMACHC Monoclonal antibody

MMACHC Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4G10A11

Cat no : 66609-1-Ig

Synonyms

Cyanocobalamin reductase / alkylcobalamin dealkylase, Cyanocobalamin reductase, cyanide-eliminating, CblC, Alkylcobalamin:glutathione S-alkyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HepG2 using 66609-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of Raji using 66609-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HEK-293 using 66609-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HeLa using 66609-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of K-562 using 66609-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of RAW 264.7 using 66609-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    IF Staining of HepG2 using 66609-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MMACHC antibody (66609-1-Ig, Clone: 4G10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MMACHC Monoclonal antibody (66609-1-Ig)

    FC experiment of HeLa using 66609-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human MMACHC (66609-1-Ig, Clone:4G10A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HEK-293 cells, HeLa cells, Raji cells, RAW 264.7 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66609-1-Ig targets MMACHC in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMACHC fusion protein Ag15001 相同性解析による交差性が予測される生物種
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Calculated molecular weight 282 aa, 32 kDa
GenBank accession numberBC006122
Gene symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_2881969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

Protocols

Product Specific Protocols
WB protocol for MMACHC antibody 66609-1-IgDownload protocol
IF protocol for MMACHC antibody 66609-1-IgDownload protocol
FC protocol for MMACHC antibody 66609-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.

Authors - Lingzhi Wang
View Article
ratWB

Mol Neurobiol

Surfeit Locus Protein 4 as a Novel Target for Therapeutic Intervention in Cerebral Ischemia-Reperfusion Injury

Authors - Wenjie Hu
View Article