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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of Raji using 66609-1-Ig (same clone as 66609-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of K-562 using 66609-1-Ig (same clone as 66609-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of RAW 264.7 using 66609-1-Ig (same clone as 66609-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HEK-293 using 66609-1-Ig (same clone as 66609-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MMACHC Monoclonal antibody (66609-1-Ig)

    WB analysis of HeLa using 66609-1-Ig (same clone as 66609-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66609-1-Ig (MMACHC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMACHC Monoclonal antibody (66609-1-Ig)

    IF Staining of HepG2 using 66609-1-Ig (same clone as 66609-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MMACHC antibody (66609-1-Ig, Clone: 4G10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MMACHC Monoclonal antibody (66609-1-Ig)

    FC experiment of HeLa using 66609-1-Ig (same clone as 66609-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human MMACHC (66609-1-Ig, Clone:4G10A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66609-1-PBS in a different storage buffer formulation.

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MMACHC Monoclonal antibody, PBS Only

MMACHC Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66609-1-PBS
Clone No.4G10A11

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Cyanocobalamin reductase / alkylcobalamin dealkylase, Cyanocobalamin reductase, cyanide-eliminating, CblC, Alkylcobalamin:glutathione S-alkyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66609-1-PBS targets MMACHC in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMACHC fusion protein Ag15001 相同性解析による交差性が予測される生物種
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Calculated molecular weight 282 aa, 32 kDa
GenBank accession numberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_2881969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4U1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.