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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP-9 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG1

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array

Range

Cytometric Bead Array: Physical level

Cat No : MP51000-1

Print datasheet

Synonyms

Validation Data Gallery

  • Cytometric bead array of U2OS cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. U2OS cells were cultured for 2 days in normal culture media or PMA (200nM, 48h) containing media. 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

    Sample test of MP51000-1

    Cytometric bead array of U2OS cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. U2OS cells were cultured for 2 days in normal culture media or PMA (200nM, 48h) containing media. 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array of A549 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. A549 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

    Sample test of MP51000-1

    Cytometric bead array of A549 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. A549 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array of MM231 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. MM231 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

    Sample test of MP51000-1

    Cytometric bead array of MM231 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. MM231 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

Tested Applications

Cytometric bead array

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP51000-1 targets MMP-9 in immunoassays as a matched antibody pair. Validated in Cytometric bead array.

Range: Physical level (Cytometric Bead Array);

Capture antibody: MMP-9 Monoclonal antibody, PBS Only (Capture) 60600-1-PBS (3C3E2). 100 μg. Concentration 1 mg/ml.

Detection antibody: MMP-9 Monoclonal antibody, PBS Only (Detector) 60600-2-PBS (1E5G7). 100 μg. Concentration 1 mg/ml.

Alternative MMP-9 matched antibody pairs: MP01003-1, MP01003-2, MP01003-3

Matched pair protein standard:

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 60600-1-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Synonyms MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9
GenBank accession numberBC006093
Gene symbol MMP-9
UniprotP14780
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 60600-2-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Synonyms MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9
GenBank accession numberBC006093
Gene symbol MMP-9
UniprotP14780
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.