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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-231 cells using MMP1 Recombinant antibody (83114-2-RR)

    WB analysis of MDA-MB-231 using 83114-2-RR (same clone as 83114-2-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 83114-2-RR (MMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83114-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP1 Recombinant antibody (83114-2-RR)

    IF Staining of HepG2 using 83114-2-RR (same clone as 83114-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP1 antibody (83114-2-RR, Clone: 230343G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83114-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MMP1 Recombinant antibody (83114-2-RR)

    FC experiment of HeLa using 83114-2-RR (same clone as 83114-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP1 Recombinant antibody (83114-2-RR, Clone:230343G1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83114-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83114-2-RR

    Affinity and Kinetic Characterization of 83114-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83114-2-RR against Human MMP1 were performed. The affinity constant is 8.01 nM.

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MMP1 Recombinant antibody, PBS Only

MMP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83114-2-PBS
Clone No.230343G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase, 230343G1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83114-2-PBS targets MMP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Calculated molecular weight 54 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC013875
Gene Symbol MMP1
Gene ID (NCBI) 4312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP03956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).