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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP1 Recombinant antibody

MMP1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230343G1

Cat no : 83114-2-RR

Synonyms

EC:3.4.24.7, CLGN, CLG, 27 kDa interstitial collagenase, 230343G1

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-231 cells using MMP1 Recombinant antibody (83114-2-RR)

    WB analysis of MDA-MB-231 using 83114-2-RR

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 83114-2-RR (MMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP1 Recombinant antibody (83114-2-RR)

    IF Staining of HepG2 using 83114-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP1 antibody (83114-2-RR, Clone: 230343G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using MMP1 Recombinant antibody (83114-2-RR)

    FC experiment of HeLa using 83114-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP1 Recombinant antibody (83114-2-RR, Clone:230343G1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83114-2-RR

    Affinity and Kinetic Characterization of 83114-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83114-2-RR against Human MMP1 were performed. The affinity constant is 8.01 nM.

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Tested Applications

Positive WB detected inMDA-MB-231 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83114-2-RR targets MMP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Calculated molecular weight 54 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC013875
Gene symbol MMP1
Gene ID (NCBI) 4312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Protocols

Product Specific Protocols
WB protocol for MMP1 antibody 83114-2-RRDownload protocol
IF protocol for MMP1 antibody 83114-2-RRDownload protocol
FC protocol for MMP1 antibody 83114-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols