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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MMP19 Polyclonal antibody

MMP19 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14244

Synonyms

matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated MMP19 Polyclonal ant (CL488-14244)

    IF Staining of SH-SY5Y using CL488-14244

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 MMP19 antibody (CL488-14244) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14244 targets MMP19 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP19 fusion protein Ag5501 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 19
Calculated molecular weight 57 kDa
Observed molecular weight 57-60 kDa
GenBank accession numberBC050368
Gene symbol MMP19
Gene ID (NCBI) 4327
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MMP19 antibody CL488-14244Download protocol
Standard Protocols
Click here to view our Standard Protocols