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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MMP2 Polyclonal antibody

MMP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10373

Synonyms

Matrix metalloproteinase 2, Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MMP2 Polyclonal anti (CL488-10373)

    IF Staining of HepG2 using CL488-10373

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MMP2 antibody (CL488-10373) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated MMP2 Polyclonal anti (CL488-10373)

    FC experiment of PC-3 using CL488-10373

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MMP2 (CL488-10373) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10373 targets MMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP2 fusion protein Ag0549 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Calculated molecular weight 72 kDa
Observed molecular weight53-72 kDa
GenBank accession numberBC002576
Gene symbol MMP2
Gene ID (NCBI) 4313
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MMP2 antibody CL488-10373Download protocol
Standard Protocols
Click here to view our Standard Protocols