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CoraLite® Plus 488-conjugated MMP3 Monoclonal antibody
MMP3 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F5A9
Cat no : CL488-66338
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66338 targets MMP3 in IF/ICC applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Reactivity | human, rat, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MMP3 fusion protein Ag12359 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Calculated molecular weight | 477 aa, 54 kDa |
| Observed molecular weight | 45-60 kDa |
| GenBank accession number | BC074869 |
| Gene symbol | MMP3 |
| Gene ID (NCBI) | 4314 |
| RRID | AB_2919311 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MMP3 antibody CL488-66338 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
