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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated MMP3 Monoclonal anti (CL488-66338)

    IF Staining of A549 using CL488-66338

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using CoraLite® Plus 488 MMP3 antibody (CL488-66338, Clone: 1F5A9 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MMP3 Monoclonal antibody

MMP3 Monoclonal Antibody for IF/ICC
Cat No. CL488-66338
Clone No.1F5A9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

IF/ICC

Synonyms

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66338 targets MMP3 in IF/ICC applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene Symbol MMP3
Gene ID (NCBI) 4314
RRIDAB_2919311
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08254
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MMP3 antibody CL488-66338Download protocol
Standard Protocols
Click here to view our Standard Protocols