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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP7 Monoclonal antibody

MMP7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2E6D6

Cat no : 67990-1-Ig

Synonyms

Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of SKOV-3 using 67990-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of HT-29 using 67990-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of COLO 320 using 67990-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of A549 using 67990-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IHC staining of human stomach cancer using 67990-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IHC staining of human stomach cancer using 67990-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inSKOV-3 cells, HT-29 cells, COLO 320 cells, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67990-1-Ig targets MMP7 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MMP7 fusion protein Ag0550 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight 29 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC003635
Gene symbol MMP7
Gene ID (NCBI) 4316
RRIDAB_2918739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MMP7 antibody 67990-1-IgDownload protocol
IHC protocol for MMP7 antibody 67990-1-IgDownload protocol
IF protocol for MMP7 antibody 67990-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols