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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP7 Monoclonal antibody, PBS Only (Detector)

MMP7 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6D6

Cat no : 67990-1-PBS

Synonyms

Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin, EC:3.4.24.23

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of SKOV-3 using 67990-1-Ig (same clone as 67990-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of HT-29 using 67990-1-Ig (same clone as 67990-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of COLO 320 using 67990-1-Ig (same clone as 67990-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MMP7 Monoclonal antibody (67990-1-Ig)

    WB analysis of A549 using 67990-1-Ig (same clone as 67990-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67990-1-Ig (MMP7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IHC staining of human stomach cancer using 67990-1-Ig (same clone as 67990-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67990-1-Ig (MMP7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Monoclonal antibody (67990-1-Ig)

    IF Staining of human pancreas cancer using 67990-1-Ig (same clone as 67990-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using MMP7 antibody (67990-1-Ig, Clone: 2E6D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67990-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50369-1

    Cytometric bead array standard curve of MP50369-1

    Cytometric bead array standard curve of MP50369-1, MMP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67990-2-PBS. Detection antibody: 67990-1-PBS. Standard:Ag0550. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67990-1-PBS targets MMP7 as part of a matched antibody pair:

MP50369-1: 67990-2-PBS capture and 67990-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MMP7 fusion protein Ag0550 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight 29 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC003635
Gene symbol MMP7
Gene ID (NCBI) 4316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.