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- KD/KO Validated
MOV10 Polyclonal antibody
MOV10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 10370-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | Hepg2 cells |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 23 publications below |
| IHC | See 1 publications below |
| IF | See 8 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
10370-1-AP targets MOV10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MOV10 fusion protein Ag0384 相同性解析による交差性が予測される生物種 |
| Full Name | Mov10, Moloney leukemia virus 10, homolog (mouse) |
| Calculated molecular weight | 114 kDa |
| Observed molecular weight | 110-115 kDa |
| GenBank accession number | BC002548 |
| Gene symbol | MOV10 |
| Gene ID (NCBI) | 4343 |
| RRID | AB_2297897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MOV10 antibody 10370-1-AP | Download protocol |
| IHC protocol for MOV10 antibody 10370-1-AP | Download protocol |
| IF protocol for MOV10 antibody 10370-1-AP | Download protocol |
| IP protocol for MOV10 antibody 10370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. | ||
Mol Cell Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change. | ||
Mol Cell MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.
| ||
Nat Struct Mol Biol Capped small RNAs and MOV10 in human hepatitis delta virus replication.
| ||
PLoS Pathog APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication. |
