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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MOV10 Polyclonal antibody

MOV10 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10370

Synonyms

KIAA1631, Helicase MOV-10, gb110, fSAP113, EC:3.6.4.13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MOV10 Polyclonal ant (CL488-10370)

    IF Staining of HepG2 using CL488-10370

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MOV10 antibody (CL488-10370) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MOV10 Polyclonal ant (CL488-10370)

    FC experiment of HeLa using CL488-10370

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MOV10 (CL488-10370) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10370 targets MOV10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOV10 fusion protein Ag0384 相同性解析による交差性が予測される生物種
Full Name Mov10, Moloney leukemia virus 10, homolog (mouse)
Calculated molecular weight 114 kDa
Observed molecular weight 110-115 kDa
GenBank accession numberBC002548
Gene symbol MOV10
Gene ID (NCBI) 4343
RRIDAB_2918975
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MOV10 antibody CL488-10370Download protocol
FC protocol for CL Plus 488 MOV10 antibody CL488-10370Download protocol
Standard Protocols
Click here to view our Standard Protocols