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MPI Monoclonal antibody

MPI Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3B10A6

Cat no : 68417-1-Ig

Synonyms

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPI Monoclonal antibody (68417-1-Ig)

    WB analysis using 68417-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rat brain tissue using MPI Monoclonal antibody (68417-1-Ig)

    WB analysis of rat brain using 68417-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MPI Monoclonal antibody (68417-1-Ig)

    WB analysis of HEK-293 using 68417-1-Ig

    WB result of MPI antibody (68417-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MPI Monoclonal antibody (68417-1-Ig)

    IP experiment of Jurkat using 68417-1-Ig

    IP result of anti-MPI (IP:68417-1-Ig, 4ug; Detection:68417-1-Ig 1:2000) with Jurkat cells lysate 2280 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPI Monoclonal antibody (68417-1-Ig)

    IHC staining of mouse brain using 68417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPI Monoclonal antibody (68417-1-Ig)

    IHC staining of mouse brain using 68417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MPI Monoclonal antibody (68417-1-Ig)

    IF Staining of NIH/3T3 using 68417-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using MPI antibody (68417-1-Ig, Clone: 3B10A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, rat brain tissue, A549 cells, LnCaP cells, Jurkat cells, K-562 cells, pig brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68417-1-Ig targets MPI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MPI fusion protein Ag5475 相同性解析による交差性が予測される生物種
Full Name mannose phosphate isomerase
Calculated molecular weight 47 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC046357
Gene symbol MPI
Gene ID (NCBI) 4351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MPI antibody 68417-1-IgDownload protocol
IHC protocol for MPI antibody 68417-1-IgDownload protocol
IF protocol for MPI antibody 68417-1-IgDownload protocol
IP protocol for MPI antibody 68417-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols