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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MPI Monoclonal antibody

MPI Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10A6

Cat no : CL488-68417

Synonyms

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated MPI Monoclonal antib (CL488-68417)

    IF Staining of NIH/3T3 using CL488-68417

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 MPI antibody (CL488-68417, Clone: 3B10A6 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68417 targets MPI in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MPI fusion protein Ag5475 相同性解析による交差性が予測される生物種
Full Name mannose phosphate isomerase
Calculated molecular weight 47 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC046357
Gene symbol MPI
Gene ID (NCBI) 4351
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MPI antibody CL488-68417Download protocol
Standard Protocols
Click here to view our Standard Protocols