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CoraLite®594-conjugated MPO Monoclonal antibody
MPO Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgA
Reactivity
human, rat
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4C11F6
Cat no : CL594-66177
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human appendicitis tissue, human liver tissue |
| Positive FC (Intra) detected in | HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL594-66177 targets MPO in IF-P, FC (Intra) applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgA |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MPO fusion protein Ag17564 相同性解析による交差性が予測される生物種 |
| Full Name | myeloperoxidase |
| Calculated molecular weight | 745 aa, 84 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC130476 |
| Gene symbol | MPO |
| Gene ID (NCBI) | 4353 |
| RRID | AB_2919960 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MPO antibody CL594-66177 | Download protocol |
| FC protocol for CL594 MPO antibody CL594-66177 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Thromb Haemost Neutrophil Extracellular Traps: Potential Prothrombotic State Markers and Therapeutic Targets for Atrial Fibrillation |
