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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MRPS17 Polyclonal antibody

MRPS17 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18881

Synonyms

S17mt, RPMS17, MRP-S17, MRPS 17, MRP S17

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MRPS17 Polyclonal an (CL488-18881)

    IF Staining of HepG2 using CL488-18881

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MRPS17 antibody (CL488-18881) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated MRPS17 Polyclonal an (CL488-18881)

    FC experiment of A549 using CL488-18881

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Mrps17 Polyclonal Antibody (CL488-18881)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18881 targets MRPS17 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS17 fusion protein Ag13414 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S17
Calculated molecular weight 130 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC054031
Gene symbol MRPS17
Gene ID (NCBI) 51373
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MRPS17 antibody CL488-18881Download protocol
FC protocol for CL Plus 488 MRPS17 antibody CL488-18881Download protocol
Standard Protocols
Click here to view our Standard Protocols