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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®488-conjugated MSH6 Monoclonal antibody (CL488-66172)

    FC experiment of HEK-293 using CL488-66172

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®488 Anti-Human MSH6 (CL488-66172, Clone:2E10B2)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  • KD/KO Validated

CoraLite®488-conjugated MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66172
Clone No.2E10B2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66172 targets MSH6 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MSH6 fusion protein Ag12645 相同性解析による交差性が予測される生物種
Full Name mutS homolog 6 (E. coli)
Calculated molecular weight 153 kDa
GenBank accession numberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
RRIDAB_2919294
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52701
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Protocols

Product Specific Protocols
FC protocol for CL488 MSH6 antibody CL488-66172Download protocol
Standard Protocols
Click here to view our Standard Protocols