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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mesothelin Monoclonal antibody

Mesothelin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3A4

Cat no : 66404-1-Ig

Synonyms

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis of HeLa using 66404-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis using 66404-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, SW 1990 cells, HGC-27 cells, MKN-45 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66404-1-Ig targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mesothelin fusion protein Ag23606 相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa, 40 kDa
GenBank accession numberBC003512
Gene symbol MSLN
Gene ID (NCBI) 10232
RRIDAB_2881778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

Protocols

Product Specific Protocols
WB protocol for Mesothelin antibody 66404-1-IgDownload protocol
IHC protocol for Mesothelin antibody 66404-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Exp Clin Cancer Res

Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET

Authors - Shengkai Xia
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 65 kd, which is as shown on the website

  • Applications: Western Blot