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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

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Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66404-1-PBS
Clone No.2E3A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66404-1-PBS targets Mesothelin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mesothelin fusion protein Ag23606 相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa, 40 kDa
GenBank accession numberBC003512
Gene Symbol MSLN
Gene ID (NCBI) 10232
RRIDAB_2881778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13421
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).