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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mesothelin Recombinant antibody, PBS Only (Detector)

Mesothelin Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240795G5

Cat no : 83744-2-PBS

Synonyms

MSLN, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor, CAK1 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mesothelin Recombinant antibody (83744-2-RR)

    WB analysis using 83744-2-RR (same clone as 83744-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00697-2

    Sandwich ELISA standard curve of MP00697-2

    Sandwich ELISA standard curve of MP00697-2, Human MSLN Recombinant Matched Antibody Pair - PBS only. 83744-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31658. 83744-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Urine of four humans was measured. The human MSLN concentration of detected samples was determined to be 923.5 pg/mL with a range of 138.7 - 1,373.8 pg/mL.

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Saliva of four humans was measured. The human MSLN concentration of detected samples was determined to be 5,273.4 pg/mL with a range of 209.0 - 16,634.9 pg/mL.

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Serum of four humans was measured. The human MSLN concentration of detected samples was determined to be 35,341.8 pg/mL with a range of 16,291.9 - 52,246.0 pg/mL.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Mesothelin Recombinant antibody (83744-2-RR)

    IF Staining of THP-1 using 83744-2-RR (same clone as 83744-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mesothelin Recombinant antibody (83744-2-RR)

    IF Staining of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00697-2

    Cytometric bead array standard curve of MP00697-2

    Cytometric bead array standard curve of MP00697-2, MSLN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-5-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00697-3

    Cytometric bead array standard curve of MP00697-3

    Cytometric bead array standard curve of MP00697-3, MSLN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-3-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83744-2-RR

    Affinity and Kinetic Characterization of 83744-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83744-2-PBS targets Mesothelin as part of a matched antibody pair:

MP00697-2: 83744-5-PBS capture and 83744-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00697-3: 83744-3-PBS capture and 83744-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight69KD
Observed molecular weight 69 kDa, 40 kDa
GenBank accession numberNM_005823
Gene symbol MSLN
Gene ID (NCBI) 10232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).