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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MSLN Recombinant antibody

MSLN Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240795G5

Cat no : 83744-2-RR

Synonyms

Mesothelin, Mesothelin, cleaved form, Megakaryocyte-potentiating factor, CAK1 antigen, CAK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSLN Recombinant antibody (83744-2-RR)

    WB analysis using 83744-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MSLN Recombinant antibody (83744-2-RR)

    IF Staining of HeLa using 83744-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83744-2-RR

    Affinity and Kinetic Characterization of 83744-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

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Tested Applications

Positive WB detected inHeLa cells, MKN-45 cells, BxPC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83744-2-RR is MSLN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight69KD
Observed molecular weight 69 kDa, 40 kDa
GenBank accession numberNM_005823
Gene symbol MSLN
Gene ID (NCBI) 10232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

Protocols

Product Specific Protocols
WB protocol for MSLN antibody 83744-2-RRDownload protocol
IF protocol for MSLN antibody 83744-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols