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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTA1 Polyclonal antibody (30545-1-AP)

    WB analysis using 30545-1-AP (same clone as 30545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTA1 Polyclonal antibody (30545-1-AP)

    WB analysis using 30545-1-AP (same clone as 30545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human skin cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human skin cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of rat cerebellum using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of rat cerebellum using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MTA1 Polyclonal antibody (30545-1-AP)

    IF Staining of MCF-7 using 30545-1-AP (same clone as 30545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MTA1 antibody (30545-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

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MTA1 Polyclonal antibody, PBS Only

MTA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30545-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30545-1-PBS targets MTA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTA1 fusion protein Ag30994 相同性解析による交差性が予測される生物種
Full Name metastasis associated 1
Calculated molecular weight 81 kDa
Observed molecular weight70-80 kDa
GenBank accession numberNM_004689
Gene Symbol MTA1
Gene ID (NCBI) 9112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13330
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Metastasis-associated protein 1 (MTA1) is a member of the MTA family which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins(PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancer.