Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-66706 targets MTAP in WB applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MTAP fusion protein Ag17954 相同性解析による交差性が予測される生物種 |
| Full Name | methylthioadenosine phosphorylase |
| Calculated molecular weight | 283 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC018625 |
| Gene Symbol | MTAP |
| Gene ID (NCBI) | 4507 |
| RRID | AB_2883844 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13126 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP MTAP antibody HRP-66706 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |