Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-55070 targets MTCO2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome c oxidase II |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 23-26 kDa |
| GenBank accession number | YP_003024029 |
| Gene Symbol | MTCO2 |
| Gene ID (NCBI) | 4513 |
| RRID | AB_2934972 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX2, also named as COII, COXII and MTCO2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to COX2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 MTCO2 antibody CL647-55070 | Download protocol |
| FC protocol for CL Plus 647 MTCO2 antibody CL647-55070 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |