MTHFD2 Monoclonal antibody
MTHFD2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B4B6
Cat no : 68524-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, LNCaP cells, HCT 116 cells, HeLa cells, HepG2 cells, K-562 cells, U2OS cellls |
Positive IHC detected in | human lung cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68524-1-Ig targets MTHFD2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MTHFD2 fusion protein Ag33605 相同性解析による交差性が予測される生物種 |
Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
Calculated molecular weight | 350 aa, 38 kDa |
Observed molecular weight | 33-38 kDa |
GenBank accession number | BC017054 |
Gene symbol | MTHFD2 |
Gene ID (NCBI) | 10797 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).
Protocols
Product Specific Protocols | |
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WB protocol for MTHFD2 antibody 68524-1-Ig | Download protocol |
IHC protocol for MTHFD2 antibody 68524-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |