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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated MTMR14 Monoclonal an (CL488-67561)

    IF Staining of mouse skeletal muscle using CL488-67561

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 MTMR14 antibody (CL488-67561, Clone: 1A8B2 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated MTMR14 Monoclonal antibody

MTMR14 Monoclonal Antibody for IF-P
Cat No. CL488-67561
Clone No.1A8B2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF-P

Synonyms

C3orf29, FLJ22405, FLJ46453, FLJ90311, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67561 targets MTMR14 in IF-P applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MTMR14 fusion protein Ag6883 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 14
Calculated molecular weight 72 kDa
Observed molecular weight74 kDa
GenBank accession numberBC050626
Gene Symbol MTMR14
Gene ID (NCBI) 64419
RRIDAB_2919501
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NCE2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MTMR14 antibody CL488-67561Download protocol
Standard Protocols
Click here to view our Standard Protocols