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  • Featured Product
  • KD/KO Validated

mTOR Monoclonal antibody

mTOR Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (8)

Applications

WB, IHC, IF/ICC, IP, ELISA, PLA

Conjugate

Unconjugated

CloneNo.

1G11A3

Cat no : 66888-1-Ig

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis using 66888-1-Ig

    Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66888-1-Ig (MTOR antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis using 66888-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis using 66888-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of HeLa using 66888-1-Ig

    WB result of mTOR antibody (66888-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of HeLa using 66888-1-Ig

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Western Blot (WB) analysis of HEK-293 cells using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of HEK-293 using 66888-1-Ig

    Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Western Blot (WB) analysis of HEK-293 cells using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of HEK-293 using 66888-1-Ig

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Western Blot (WB) analysis of various lysates using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis using 66888-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of mouse brain using 66888-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of ROS1728 using 66888-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using mTOR Monoclonal antibody (66888-1-Ig)

    WB analysis of rat brain using 66888-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using mTOR Monoclonal antibody (66888-1-Ig)

    IP experiment of HeLa using 66888-1-Ig

    IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using mTOR Monoclonal antibody (66888-1-Ig)

    IP experiment of HeLa using 66888-1-Ig

    IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human colon cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human colon cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human breast cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human breast cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human liver cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using mTOR Monoclonal antibody (66888-1-Ig)

    IHC staining of human liver cancer using 66888-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Monoclonal antibody (66888-1-Ig)

    IF Staining of HepG2 using 66888-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Monoclonal antibody (66888-1-Ig)

    IF Staining of HepG2 using 66888-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using mTOR Monoclonal antibody (66888-1-Ig)

    IF Staining of HeLa using 66888-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Monoclonal antibody (66888-1-Ig)

    IF Staining of HepG2 using 66888-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66888-1-Ig targets mTOR in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat, pig, chicken, hamster, goat, zebra finches
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight 250-289 kDa
GenBank accession numberNM_004958
Gene symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_2882219
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

Protocols

Product Specific Protocols
WB protocol for mTOR antibody 66888-1-IgDownload protocol
IHC protocol for mTOR antibody 66888-1-IgDownload protocol
IF protocol for mTOR antibody 66888-1-IgDownload protocol
IP protocol for mTOR antibody 66888-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
View Article
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
humanWB,IP

Mol Cell

Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells

Authors - Lixin He
View Article
humanWB

Mol Cancer

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.

Authors - Xiaomin Li
View Article
humanWB

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
View Article
mousePLA

Nat Commun

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.

Authors - Xin Zhou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sun (Verified Customer) (01-05-2023)

strong band showed up around 200kDa region in HEK293T cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Macarena (Verified Customer) (10-07-2022)

excellent band. Very concentrated.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: cell lines
FH

Rachel (Verified Customer) (05-24-2021)

Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: PC3 cells
mTOR Antibody Western Blot validation (1/50 dilution) in PC3 cells (Cat no:66888-1-Ig)