- Featured Product
- KD/KO Validated
CoraLite®594-conjugated mTOR Monoclonal antibody
mTOR Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (2)
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G11A3
Cat no : CL594-66888
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 2 publications below |
Product Information
CL594-66888 targets mTOR in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種 |
| Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
| Calculated molecular weight | 289 kDa |
| Observed molecular weight | 250-289 kDa |
| GenBank accession number | NM_004958 |
| Gene symbol | mTOR |
| Gene ID (NCBI) | 2475 |
| RRID | AB_2883633 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 mTOR antibody CL594-66888 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genet Med De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood | ||
bioRxiv Monocytes reprogrammed by 4-PBA potently contribute to the resolution of inflammation and atherosclerosis |
