MUC2 Polyclonal antibody

MUC2 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27675-1-AP

Synonyms

SMUC, Mucin 2, MUC-2, MUC 2, MLP


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Tested Applications

Positive IHC detected inhuman colon tissue, human pancreas cancer tissue, mouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue, mouse colon tissue, rat small intestine tissue
Positive IF/ICC detected inHepG2 cells, SW480 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27675-1-AP targets MUC2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, duck, mosquito
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC2 fusion protein Ag25800 相同性解析による交差性が予測される生物種
Full Name mucin 2, oligomeric mucus/gel-forming
Calculated molecular weight 540 kDa
GenBank accession numberM94132
Gene symbol MUC2
Gene ID (NCBI) 4583
RRIDAB_2880943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

Protocols

Product Specific Protocols
IHC protocol for MUC2 antibody 27675-1-APDownload protocol
IF protocol for MUC2 antibody 27675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway

Authors - Yanan Zhang
mouseIF

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
mouseIF

Pharmacol Res

λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity

Authors - Wei Wu
mouseIF

NPJ Biofilms Microbiomes

Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota

Authors - Tong Zhao
humanIHC

Chemosphere

A possible but unrecognized risk of acceptable daily intake dose triazole pesticides exposure-bile acid disturbance induced pharmacokinetic changes of oral medication

Authors - Yujing Xu
mouseIF

Gut Microbes

Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier.

Authors - Li Ge

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-24-2022)

This is great Antibody. it has no background

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: colon tissue and colon cancer cells line