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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MUC2 Polyclonal antibody

MUC2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27675

Synonyms

Intestinal mucin 2, MLP, MUC 2, MUC2, Mucin 2, SMUC

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MUC2 Polyclonal anti (CL488-27675)

    IF Staining of HepG2 using CL488-27675

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MUC2 antibody (CL488-27675) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-27675 targets MUC2 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC2 fusion protein Ag25800 相同性解析による交差性が予測される生物種
Full Name mucin 2, oligomeric mucus/gel-forming
Calculated molecular weight 540 kDa
GenBank accession numberM94132
Gene symbol MUC2
Gene ID (NCBI) 4583
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MUC2 antibody CL488-27675Download protocol
Standard Protocols
Click here to view our Standard Protocols