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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of HEK-293 using 60353-1-Ig (same clone as 60353-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of A375 using 60353-1-Ig (same clone as 60353-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of Jurkat using 60353-1-Ig (same clone as 60353-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MUM1 Monoclonal antibody (60353-1-Ig)

    IF Staining of HeLa using 60353-1-Ig (same clone as 60353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    IF Staining of A375 using 60353-1-Ig (same clone as 60353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 60353-1-Ig (MUM1 antibody), at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60353-1-PBS in a different storage buffer formulation.

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MUM1 Monoclonal antibody, PBS Only

MUM1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60353-1-PBS
Clone No.3B1D2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60353-1-PBS targets MUM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MUM1 fusion protein Ag3371 相同性解析による交差性が予測される生物種
Full Name melanoma associated antigen (mutated) 1
Calculated molecular weight 18 kDa, 79 kDa
Observed molecular weight 78-80 kDa
GenBank accession numberBC008098
Gene Symbol MUM1
Gene ID (NCBI) 84939
RRIDAB_2881462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ2TAK8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.