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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MUM1 Monoclonal antibody

MUM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B1D2

Cat no : 60353-1-Ig

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of HEK-293 using 60353-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of A375 using 60353-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MUM1 Monoclonal antibody (60353-1-Ig)

    WB analysis of Jurkat using 60353-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MUM1 Monoclonal antibody (60353-1-Ig)

    IF Staining of HeLa using 60353-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MUM1 Monoclonal antibody (60353-1-Ig)

    IF Staining of A375 using 60353-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 60353-1-Ig (MUM1 antibody), at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60353-1-Ig targets MUM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MUM1 fusion protein Ag3371 相同性解析による交差性が予測される生物種
Full Name melanoma associated antigen (mutated) 1
Calculated molecular weight 18 kDa, 79 kDa
Observed molecular weight 78-80 kDa
GenBank accession numberBC008098
Gene symbol MUM1
Gene ID (NCBI) 84939
RRIDAB_2881462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Protocols

Product Specific Protocols
WB protocol for MUM1 antibody 60353-1-IgDownload protocol
IF protocol for MUM1 antibody 60353-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.

Authors - Zhou Qi
View Article