Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60353 targets MUM1 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MUM1 fusion protein Ag3371 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma associated antigen (mutated) 1 |
| Calculated molecular weight | 18 kDa, 79 kDa |
| Observed molecular weight | 78-80 kDa |
| GenBank accession number | BC008098 |
| Gene Symbol | MUM1 |
| Gene ID (NCBI) | 84939 |
| RRID | AB_2919923 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q2TAK8 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MUM1 antibody CL594-60353 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |